| Summary: | 碩士 === 國立東華大學 === 生物技術研究所 === 92 === Anthraquinones existed in many herb plants and metabolites of microorganisms. Their biological activities include laxative, antibacterial, antifungal, antiviral and anticancer. In this study, two kinds of anthraquinones derivatives, chrysophanol and pachybasin were isolated from Trichoderma harzianum strain ETS 323. Though both of them are known compounds, they were first isolated from T.harzianum strain ETS 323. We were hoping to understand the function of the two compounds on biological control of plant diseases. The spores of T.harzianum strain ETS 323 were collected for 6 days inculation on PDA and inoculated on sugarcane bagasse. After 8 days sugarcane bagasse was extracted with ethyl acetate. The extract was analyzed by thin layer chromatography to test the appropriate condition for silica gel column chromatography. There were two compounds, yellow material and orange material, obtained by silica gel column chromatography. The structure of orange material was identified as chrysophanol by the X-ray diffraction. The structure of yellow material was identified as pachybasin by NMR. Then the effects on germination and number of lateral root of plant seeds, number of spores germination and growth of T.harzianum strain ETS 323, T.virens and plant pathogenic fungi and bacteria. ( A.brassicicola, P.aphanidermatum, R. solani, P.capsici, E.carotovora) were tested. For plant seeds germination, the result showed that pepper, sweet pepper and eggplant and number of lateral root of broccoli, rape and tomato were promoted when treating with chrysophanol by using Ducan’s test. Pachybasin promoted number of lateral root of rape and tomato. In T.harzianum strain ETS 323, T.virens and pathogenic fungi and bacteria, chrysophanol and pachybasin inhibited mycelium germination of R.solani, but not others. In summary, the study first reported chrysophanol and pachybasin from T. harzianum strain ETS 323. They improved some plant seeds germination rate and number of lateral root. Also they inhibited mycelium germination of R. solani. In the future, other plant seeds and locally born phytopathogenic fungi and bacteria will be extensively and systemic and more knowledge of biological control.
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