Computer Simulation Analysis of Microchannel for a Continuous-Flow PCR Chip

碩士 === 國立嘉義大學 === 生物機電工程學系碩士班 === 92 === The purpose of this study is to analyze the model of a continuous-Flow Polymerase Chain Reaction (PCR) chip with CoventorWare software. The main idea was to compare the effects of different cross sections of channel and find out the better one for...

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Bibliographic Details
Main Authors: Pao-Chia Pan, 潘保嘉
Other Authors: Chyung Ay
Format: Others
Language:zh-TW
Published: 2004
Online Access:http://ndltd.ncl.edu.tw/handle/94956897563649817243
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Summary:碩士 === 國立嘉義大學 === 生物機電工程學系碩士班 === 92 === The purpose of this study is to analyze the model of a continuous-Flow Polymerase Chain Reaction (PCR) chip with CoventorWare software. The main idea was to compare the effects of different cross sections of channel and find out the better one for heat transfer. It offered the ability of fast heating and cooling rate when the DNA duplication process happened. Besides, the Top-down and Bottom-up methods to draw relative parameter curves of model was applied on this study. First of all, simulated the simple channel model in the literature to verify reliability of the software. The result showed that the pressure drop of channel was non-linear. When the central temperature of microchannel increased to 17℃ in the wall, the time to increase temperature in CoventorWare is 4 ms and ANSYS is 11 ms. These fitted with that the time to be need in the literature less than 100 ms. Finally, the detail behavior of microchannel was designed and analyzed by Bottom-up method. The fluidic characteristics of D section, round section, rectangular section, semicircular section and trapezoid section of microchannel were found. Then the chip system by Top-down method was simulated. The result showed that the performance of flow rate and flow time in round section of microchannel is better than it in others section of microchannel. This result is obtained the same with Bottom-up method. When the central temperature of fluid was increased from 0℃ to 30℃, the least time to be need is 2.7 ms in D section; under the pressure at 2.727×10-4 Mpa, the flow rate at 2.3×10-7 μm3/s and the flow resistance at 1.18×10-11 MPa in round section, has the best performance than any other sections. If the consideration were under by various aspects, total performance of trapezoid section of microchannel is only inferior to that of round section and D section. The central temperature of trapezoid section was increased to 30℃ needs 2.99 ms, the flow rate is 2.04×10-7 μm3/s and the flow resistance is 1.33×10-11 MPa. This form of section of microchannel is a good choice for future design.