Evaluation of genetic variation of desert rose(adenium obesum) varieties with inter simple sequence repeat markers

• Main Author: 周美君
• Other Authors: Chu-Tsai Tsai
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/33496217277045968580

碩士 === 國立嘉義大學 === 生物科技研究所 === 92 === Desert rose, Adenium obesum, is an evergreen and semi-deciduous shrub and belongs to Apocynaceae. Most of the cultivated clones were introduced from Arab, Thailand and America. In this study, 35 desert rose variety were evaluated using ISSR (inter simple sequence repeat) molecular markers. The samples comprised 3 clones of Arab, 7 clones of Thailand, 2 clones of America, and 23 hybrid clones between Thailand and America. Objectives were to assess genetic similarity among the varieties. There were 161 polymorphic bands produced by 15 selected ISSR primers. The result of ISSR suggested that among the dinucleotides motifs, the poly(AC)n motif was more abundant and gave the largest number of polymorphism. Genetic similarity among 35 samples was detected from 0% to 88% on ISSR marker basis. The cluster analysis of genetic similarity by UPGMA (Unweighted Pair-group Mean Arithmetic) revealed that four major groups could be recognized. The 3 clones of Arab had larger genetic similarity than others, thus they composed a small group in the clustering tree. This quick and reliable molecular tool is useful in selecting the best parents and obtaining new combinations.