| Summary: | 碩士 === 國立中央大學 === 生命科學研究所 === 92 === Abstract
In order to understand the mechanism of plant crop heat stress, we
screened a mutant that is difficult to tolerant heat stress called “hit1”
(heat intolerance) inducible by chemical mutagenesis, EMS, causing a
point mutation. Forward genetic is one of functional genetics approaches,
and it is underlying a mutant with a desirable genetic phenotype to an
identified mutation in a gene and to analysis specific gene’s molecular
biology function. We use forward genetic to approach “hit1” that was a
point mutation, so we want a cloning of sequencing mutant-define gene
by map-based coloning (MBC). A hit1 mutant with Columbia ecotype (P1)
and a wild type with Landsberg erect ecotype (P2) were parent to get
recombinant inbred lines (F2). It has hit1 mutant specific phenotype lines
of F2 generation that is a segregation population to provide hit1 genetic
mapping. The definition of hit1 mutant phenotype was death at 37℃ in 4
days to screen mutants from F2 generation as a segregation population
then make hit1 gene genetic mapping, and we designed 26 pairs primers
of simple sequence length polymorphism (SSLP) markers and 7 pairs
primers of cleavage amplify polymorphisms (CAPS) markers for genetic
analysis. We suggest that hit1 gene locus in AGI 18767.3kb ~ 18810.2kb,
total 42.9kb regions, and approach the fine-scale mapping of MBC.
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