Aptamer-Based Probes for Carbohydrate Antigens and Its Application

• Main Authors: Wei-Li Lu, 呂偉立
• Other Authors: none
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/41064890127552474651

碩士 === 國立中央大學 === 化學研究所 === 92 === Cell surface carbohydrates have been shown to play essential biological roles in divers biological phenomena such as molecular recognition, used the carbohydrate antigen （ex. Tn antigen found in gastrointestinal tissues） to distinguish cancer cell with normal cell. Then used Tn antigen as target molecular in the SELEX（Systemic Evolution of Ligands by EXponential Enrichments）, found the aptamers（isolation of DNA ligands out of the random sequence pool）. The aptamers have high affinity and selectivity to Tn antigen. The aptamers can be applied to develop sensor for Tn antigen. We wish the sensor could be used to diagnostic cancer early.