Effects of Protective Drugs on Gastric Hemorrhagic Ulcers in Atherosclerotic Rats

• Main Authors: Wen-Hsein Chen, 陳文獻
• Other Authors: Chan-Lu Hung
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/33611680331356755111

碩士 === 國立成功大學 === 藥理學研究所 === 92 === Abstract 　　Calcium and cholesterol play an important role in the formation of atherosclerosis. Feeding the mixture of calciferol and cholesterol induces the atherosclerosis in rats. This atherosclerotic aorta was characterized by medial calcification and intimal cell proliferation. However, it is unknown whether the mixture of cholesterol and calciferol also causes gastric hemorrhage and mucosal damage. The aim of our study is observing the gastric oxidative stress and mucosal damage induced by the mixture of calciferol and cholesterol, and we evaluated the protective effects of several drugs. The effects of these drugs were significantly attenuated in the VD+CHOL as compared with the control. Male Wistar rats were challenged intragastrically with 1ml vegetable oil containing calciferol (4.5mg/kg) and cholesterol (22.5mg/kg), once daily and for 9 days. We fed normal rats with vegetable oil alone. During gastric surgery, rat stomachs were irrigated with physiological acid solution or normal saline for 3hrs. After that rats were sacrificed. We observed the increased level of acid back-diffusion, mucosal lipid peroxide content and hemorrhage ulcer area, and the decreased mucosal glutathione content in atherosclerotic rats. Besides, there was a high correlation between mucosal lipid peroxide levels and ulcer areas. Daily feeding clofibrate (300mg/kg), verapamil (20mg/kg), lovastatin (10mg/kg) or lysozyme (500mg/kg) effectively protected gastric mucosa in atherosclerotic rats. In conclusion, the mixture of calciferol and cholesterol may be produced gastric oxidative stress, which is an important factor in the formation of hemorrhagic ulcer in atherosclerotic rats.