| Summary: | 碩士 === 國立成功大學 === 環境醫學研究所 === 92 === Trans-trans-2,4-decadienal (ttDDE) is a widespread ��,��-unsaturated aldehyde found in food, water, and environmental pollutants. It is also the major part (51.6%) in neutral fraction of methanolic extract from fumes of heating peanut oil and is shown to have strongest mutagenicity. Data collected from an epidemiological case-control study has been suspected that exposure to fumes emitted from cooking oils when not reduced by an extractor appeared to be an important risk factor for lung cancer in Taiwanese woman nonsmokers. The objective of this work is to use proteomic approaches to study how this toxic chemical compound may alter the proteome in BEAS-2B normal human bronchial epithelium cells. The differences in the levels of proteins extracted from BEAS-2B cells with and without exposure to ttDDE, were investigated using two-dimensional gel electrophoresis (2D-GE) display. More than 600 protein spots were visualized after silver staining. The comparison between 2D-GE maps with ttDDE treated or controlled showed alterations of 47 proteins, of which 35 were up-regulated and 12 were down-regulated. The identities of the proteins with altered levels were determined by liquid chromatography-mass spectrometry analysis of in-gel tryptic digests and protein sequence database search. Thirty-five proteins were identified. These proteins were further explored using the bioinformatics tools from the Expert Protein Analysis System (ExPASy) for their cellular locations and functions. Among these proteins, glutathione S-transferase (GST), NAD-dependent alcohol dehydrogenase (ADH), may play roles in ttDDE detoxification pathways. Glutathione reductase (GR), GST, thioredoxin-like protein, thioredoxin (TRX), DJ-1 protein, cofilin, ribonuclease inhibitor and serum albumin precursor may be involved in oxidative stress induced by ttDDE treatment. Besides, Retinoblastoma binding protein 7 (RBBP7) is of special interest since it is also found in pleural effusion from lung cancer patients.
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