Molecular cloning and characterization of an E-type CDK and its interacting cyclin from Arabidopsis thaliana

• Main Authors: Szu-Yuan Pu, 蒲思源
• Other Authors: Hao-Jen Huang
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/41011986721070467548

碩士 === 國立成功大學 === 生物學系碩博士班 === 92 === 　　Cyclin dependent kinase is defined as its activation requires the binding of cyclin partner. In animal, 9 groups of CDK have been identified and named CDK1-CDK9. The CDK7, CDK8, and CDK9 have been known to be able to phosphorylate the C-terminal domain (CTD) of RNA polymerase II (RNAPII) or a variety of transcription factors. Therefore, these CDK are though to be involved in gene expression regulation. In plants, 5 groups of CDK have been identified and the nomenclature CDKA-E was adopted. Comparing with the A- and B- type plant CDK which were extensively studied, relevant research for other pant CDK is lacking. 　　In present study, we isolated an E-type CDK, AtCDKE, and its interacting cyclin, ATCYCC1, from Arabidopsis thaliana. Tissue specific analysis shows AtCDKE was expressed in leaves and flowers, whereas AtCYCC1 proteins were apparently detected in flowers. The subcellular localization of both proteins were also investigated by fusing them with a green fluorescence protein (GFP). While AtCDKE is loalized in nucleus, the AtCYCC1 could be detected in either nuclear or cytoplasmic regions. Such results suggested that AtCDKE-AtCYCC1 complex is involved in the regulation of flower development by exerting its effect on transcriptional control. Moreover, DNA-gel blot analysis shows that the coding region of AtCDKE is methylated. DNA methylation has been known to be involved in the regulation of many flower-related genes’ expression. Recently reports also indicated that AtCDKE controls the development of flowers. Therefore, we suggests that AtCDKE-AtCYCC1 complex is involved in floral development and regulated co-ordinately with other flower-related genes by DNA methylation.