The in vivo and in vitro Anti-tumor Study of Magnetic Nano-Particles Coated with Anti-tumor Drug Doxorubicin

• Main Authors: Lun-Chieh Chiu, 邱崙捷
• Other Authors: Shyh-Yu Shaw
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/76833189468190593681

碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 92 === In our study, we synthesized magnetic nano-particles coated with an anti-cancer medicine doxorubicin to form three new-types magnetic nano anti-cancer medicine. By magnetic leading, the magnetic nano-particles can be targeted to the tumor site for therapy. The advantages are increasing drug concentration at the tumor and lowering drug concentration in the other region of body to avoid the significant side effect of chemical drugs. Our purpose is examining the in vivo and in vitro activity of this new-type anti-cancer medicine. In vitro activity experiment was performed on murine bladder tumor cell (MBT-2) and the survival cell was quantitated by MTT assay. The activity of magnetic nano-particles coated with doxorubicin were compared to free magnetic nano-particles and free doxorubicin. We found that magnetic nano-particle coated with doxorubicin exhibits activity similar to free doxorubicin but the magnetic nano-particle alone dosen’t show any activity. Besides, when we provide a external magnetic field to target the magnetic nano-particles and we found that magnetic nano-particles coated with doxorubicin can be targeted and killed the cancer cell. But there are no observable toxicity in the same test on normal cell line COS-7. In In vivo experiment, we first induced tumor formation in mice, and then implanted a small magnetic in tumor. Then we injected magnetic nano-particles with doxorubicin into the tumor. We found that magnetic nano-particles coated with doxorubicin can be target to the tumor site and kill the tumor cells.