Investigation of fermentation strategy in produced recombinant protein by Escherichia coli.

• Main Authors: Po-Shun Ke, 柯博順
• Other Authors: T.-L. Chen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/b248xu

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 92 === 　　A fermentation strategy for maintaining plasmid stability was investigated in this study. Since plasmid loss occurs during cell fission, a two-stage fermentation method for decreasing the probability of cell fission was proposed accordingly. At the first stage, high cell density was achieved by culturing the cells in the growth medium. At the second stage, the production of creatinase was produced by feeding the fresh producing medium with a appropriate concentration of isopropyl-β-D–thiogalactopyranoside (IPTG). The timing of cell mass doubled implied that the majority of cells had experienced one cycle of cell fission and new generation of cells were born. Therefore, the maintenance of plasmid stability could be achieved by decreasing the occurrence of cell fission, which can be done by adjusting the adding of producing medium for controlling the increase of cell mass after induction.