The evaluation of phenol degradation by immobilization of Pseudomonas putida onto chitosan beads

• Main Authors: Shen-Hsiu Hung, 洪聖修
• Other Authors: Jui-Che Lin
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/jg9ux9

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 92 === 　　In this study, we used chitosan lactic acid solution (CLS) and sodium tripolyphosphate (TPP) to immobilize the Pseudomonas putida by physical iontropic gelation and regarded it as a biocatalyst for the biodegradation of phenol. 　　From the results, our conclusions are as followings： (1)The Pseudomonas putida can be acclimatized by the phenol and have well adaptability. (2)The Pseudomonas putida stays alive in the process of immobilization according to the experimental results of the extended direct contact with CLS and TPP. (3)Owing to the effect of the low temperature, the concentration of chitosan could be reduced in the immobilization process. (4)A greater amount of immobilized Pseudomonas putida and/or a higher concentration of gelling agent can lead to a faster phenol degradation rate. 　　In this study, the immobilized chitosan beads embed by 3×10^9 CFU of the bacterials and gelated with 1 wt% of tripolyphosphate have the highest phenol degradation efficiency. This can be attributed to the residual cells from the washing, the cells fallen from the external surface of the beads due to vibration and the cells immobilized onto the chitosan beads.