| Summary: | 碩士 === 國立成功大學 === 工程科學系碩博士班 === 92 === This study was focusing on developing microchips for rapid DNA cell lysis to replace the traditional method that has the disadvantage in long time process. The prototype lysis chip was fabricated by microfabrication technology and verified using a real time PCR system. The lysis efficiency using the lysis chip was lower than that of the conventional method. In order to improve the design and understand the parameters which affected the lysis efficiency, CFD-RC software was used to understand the temperature distribution by analyzing the electric-thermal-fluid coupled-fields. The conventional cell lysis process used lysis enzyme (microLYSIS) under the thermal cycle at 96�aC, 2 min and 65�aC, 4 min for three cycles. The lysis chip was designed in flowing through and passing different temperature zones to achieve three thermal cycles. So, to find the appropriate mixture injection velocity is necessary. By using CFD-RC coupled-field analysis to simulate the prototype chip, the results showed that the efficiency was low due to that only the third cycle could approach lysis temperature. The parameters which affected the lysis efficiency were electrode geometry, injecting velocity and microchannel’s height. When applying 0.6 voltage with 0.1 mm/s of injecting velocity, the simulation data showed all thermal cycles reached lysis temperature requirement. For the second type of chip, the preheating electrodes were added, therefore, the applied voltage was reduced to 0.49V and increased the injection velocity to 0.2 mm/s. The coupled-field analysis can provide a detailed information in designing the lysis chip for further improvement in efficiency.
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