Study of Micro-Chip for Electro-Immunoassay in Real-Time

• Main Authors: Kai-Chun Su, 蘇凱群
• Other Authors: Yu-Cheng Lin
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/h4za7b

碩士 === 國立成功大學 === 工程科學系碩博士班 === 92 === 　　In this study, a novel Micro-Electrode Biochip for Immunoassay was designed and fabricated by using MEMS (Micro Electro-Mechanical Systems) technique. The characteristics of Micro-Electrode Biochip with a capacity of efficient informative nano-samples had been developed for the purpose of decreasing reaction time and the cost. On the Biochip, we were devoted to the study of the sensitivity of detection limit and took advantages of traditional ELISA in time. 　　Following the skin effect from Maxwell equation, we analyzed the signals from the electrodes on the chip. Measured impedance changes in frequency domain would help us to identify the result of Electro-Immunoassay. In order to improve the reaction and economize on time, conjugated with Au nano-particles was adopted to amplify the output signal of impedance measurement and distinguish the specific antigen easily and rapidly. Four parameters, phase, impedance, resistance and reactance, of electric properties under different testing voltages had been discussed to identify in the analysis of frequency/impedance spectroscopy. 　　Finally, we had found out some interest results in characteristic curves of electric signals. The reaction between antibody and antigen in real time could be detected in electrical measurement through high frequency domain. Furthermore, the detection limit in the concentration of antigen was 10-8 g per milliliter. In another hand, Micro-Electrode Biochip conjugated with Nano-Au (13 nm) for Electro-Immunoassay was conveniently in signal discrimination. The results of this study had been confirmed excellent influences in Immuno-topic.