Expression of matrix metalloproteinase-9 (MMP-9) in the callus before fracture-reduction in the dog

• Main Author: 陳德舉
• Other Authors: 王俊秀
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/85992002643201721656

碩士 === 國立中興大學 === 獸醫學系 === 92 === Bone healing can be divided into three phases, including the inflammation phase, the reparative phase and remodeling phase. Matrix metalloproteinase-9 (MMP-9) lyses the extracellular matrix in a fracture state. In inflammation phase, the high expression of MMP-9 is contributive to the migration of the inflammation cells resulted from degrading extracellular matrix. In addition, the high expression of MMP-9 is conducive to remodeling of bony callus during remodeling phase. This study used zymography to detect the difference of MMP-9 activity in bony callus at those distinct phases before fracture-reduction and also coordinated with the classification of histopathology. Zymography samples were diluted to 1/500 after extracted from extracellular proteinase. Thirty-one samples of bony callus before fracture-reduction were obtained by surgical intervention in Veterinary Teaching Hospital of National Chung-Hsing University. The result of this studying reveals that the zymography displayed a clear band on 92KD in samples of the bony callus of the inflammation phase and the remodeling phase, but not the reparative phase. Through the statistical analysis, it appeared a high relation (p < .05) between the expression of MMP-9 and the duration from fracture to reduction. However, the weight, age, fracture shape and fracture location did not reveal high relation with the expression of MMP-9. Continues to trace the bone fracture cases, we found that the recovery of post-surgery and the duration from fracture to reduction were not related.