| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 92 === This study investigated the bacteria flora in the genital tract of mares in Taiwan. From June 2001 to July 2002, thirty healthy mares in Middle and Southern Taiwan were used for this investigation. Swab sampling of uterus, vestibule, clitoral fossa and clitoral sinus for bacteriological culture were performed on the 30 clinically normal mares during their diestrus period. The swab specimens were inoculated in different incubation atmospheres, including aerobic, capnophilic and anaerobic conditions, respectively. The isolates were purified and identified by methods of GFB-14E, Biolog MicroStation and 16S rDNA sequencing. The result indicated that a total of 240 strains of bacteria were isolated and identified, including 4, 30, 90 and 111 strains from the 4 collected sites, respectively. The isolated rate were 10.0% (3/30)、66.7% (20/30)、100% (30/30) and 100% (30/30) from endometrial, vestibule, clitoral fossa and clitoral sinus, respectively. Pseudomonas aeruginosa (3.3%), Klebsiella pneumoniae (3.3%), Streptococcus zooepidemicus (13.3%), and Escherichia coli (40.0%), which may possibly reduce endometritis, were isolated from the genital tract of clinically health mares. Taylorella equigenitalis was not found in this investigation. There were more bacteria isolated from the uterus in the uterus in the older and multiparous mares. The vestibule harbored more bacteria in the multipara than in those without breeding record (21 strains/10 mares vs. 7 strains /6 mares). The amount of clitoral bacteria has no difference between age and breeding record (101 strains /16 mares vs. 60 strains /8 mares). The isolated rate of potential uterine pathogens have no difference between age and breeding record (7/16, 44 ％ vs.4/8, 50 ％). These results from this investigation in mares in Middle and Southern Taiwan demonstrated that the outer reproductive sites in mares supported the growth of organisms, including potential uterine pathogens. Thus, it is important to prevent transmitting the venereal disease of horses in the breeding season.
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