| Summary: | 碩士 === 國立中興大學 === 食品科學系 === 92 === Abstract
The aim of this study was to investigate the hepatic protection and antioxidation of water extract from Du-Zhong (Eucommia ulmoides Oliv.) leaves (WEDZ) (including its active compounds: chlorogenic acid and protocatechuic acid) and water extract of Cassia tora L. (WECT) against carbon tetrachloride (CCl4) induced hepatic damage in rats.
The oral treatment with WEDZ (0.1, 0.5 and 1.0 g/kg rat) for 28 consecutive days and the administration of CCl4 (0.5 mL/rat, 20%) induced hepatic damage in rats. It could find that CCl4 decrease the weight grain rate and increase the relative organ weights of liver and kidney, and treatment with WEDZ could significantly (p < 0.05) increase weight grain rate and decrease the relative organ weight of liver and kidney. In serum biochemical parameters, CCl4 induced rats liver damage could significantly (p < 0.05) increase the GOT, GPT, LDH, ALP, -GT, BUN, TBil, Chol and TG level as compared with normal control in serum. Treatment with WEDZ and its active compounds could decrease the GOT, GPT, LDH, ALP, -GT, BUN and TG level when compared with negative control. In antioxidant activity evaluation, administration of CCl4 induced rats liver damage caused the GSH content significantly (p < 0.05) decreased and MDA content increased (p < 0.05) both in liver and serum as compared with normal control. Treatment with WEDZ and its active compounds also could significantly increase (p < 0.05) the GSH content and decreased the MDA content both in liver and serum. Administration of WEDZ and its active compounds could increase the activites of GPx, GRd, GST and SOD, and the trolox equivalent antioxidant capacity (TEAC) in serum. Liver histopathology showed that WEDZ and its active compounds reduced the incidence of liver lesions including hepatic cells cloudy swelling, lymphocytes infiltration, cytoplasmic vacuolization, hepatic necrosis and fibrous connective tissue proliferated induced by CCl4 in rats.
The administration of CCl4 (0.5 mL/rat, 20%) induced hepatic damage could decrease the weight grain rate and increase the liver weight in rats. Administration of WECT could significantly (p < 0.05) increase the weight grain rate, but no significant different (p > 0.05) in rats organ weight. In serum biochemical parameters, CCl4 induced rats liver damage could significantly (p < 0.05) increase the GOT, GPT and LDH activities and TBil, Crea and Chol content as compared with normal control in serum. Treatment with WECT could significantly (p < 0.05) decrease the GOT, GPT and LDH activities, and decrease the Crea content. WECT could decrease the MDA content and increase GSH content both in liver and serum induced by CCl4. WECT could significantly (p < 0.05) increase the activites of GPx, GRd, GST, Cat and SOD decreased by CCl4. WETC could significantly (p < 0.05) increase the trolox equivalent antioxidant capacity (TEAC) in serum. Liver histopathology showed that WECT reduced the incidence of liver lesions including cloudy swelling, cytoplasmic vacuolization, ballooning degeneration and necrotic cell formation induced by CCl4 in rats.
The data suggest that oral administration with WEDZ and WECT for 28 consecutive days could significant decrease the hepatic damage level induced by CCl4 in rats. Moreover, WEDZ and WECT could increase the activites of antioxidant enzymes in liver and increase the total antioxidant capacity decrease by CCl4 in vivo to protect the liver damage.
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