| Summary: | 碩士 === 國立中興大學 === 食品科學系 === 92 === In this study, a rapid method for the isolation of compounds of Rhei Rhizoma Radix, identification of the structures of these compounds with NMR and MS, and bioactivities of the compounds were studies.
Rhei Rhizoma Radix was extracted with methanol, and was then subjected to treat with water by four methods, of which stirring with water at room temperature, stirring with boiling water, refluxing with 98℃ water, and batch treating with 98℃ water. The batch treating with 98℃ water for three times obtained good separation. The precipitate extracted with glacial acetic acid, then the supernatant was separated by preparative C18-HPLC. Nine fractions were obtained, of which five fractions giving high recoveries and purity were collected, and the structures and bioactivities of theses five fractions were studied.
Structures of five purified fractions were identified with NMR and MS. The five compounds were identified as (1), 2-O-cinnamoyl-1-O- galloyl-β-D-glucopyranoside, (2), 2-O-cinnamoyl-1,6-di-O-galloyl-β-D- glucopyranoside, (3), chrysophanol-8-O--D-glucopyranoside, (4), chrysophanol-1-O--D-glucopyranoside and (5), emodin-6-O--D- glucopyranoside.
Anitimicrobial and antiviral activities of these five compounds were examined. Compound (1) ~ (5) exhibited strong anitimicrobial activities against Staphylococcus aureus and Helicobacter pylori with 0.24 ~ 0.96 mg/mL and 0.8~12.8 mg/mL of MICs, respectively. Compound (1) ~ (4) showed anti-herpes simplex virus activities, of which compound 4 exhibited the highest anti-HSV-1 activity with 7.70 of SI value, and with descending order by compound (1) and (3). Furthermore, time of addition assay was examined. Compound (1), (3) and (4) didn’t observe prevented effect of viral infection, but showed inhibitory ability of viral adsorption and replication. After 24 h of viral infection, inhibitory activities of compound (1), (3) and (4) still observed. Of which after 24 h of infection of HSV-1, 100 % inhibition of replication of compound (4) was found at the concentration of 75 and 100 μg/mL.
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