Cytotoxicity Evaluation of Camptothecin and its Solid Lipid Nanoparticles in Human Hepatoma Cell

• Main Authors: Jia-Wen Shih, 施家雯
• Other Authors: Tong-Rong Tsai
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/66951437381652304577

碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 92 === In this study, we used Camptothecin (CA) as a model drug for preparation in hot homogenization. camptothecin is a natural, water-insolube alkaloid. 25 factorial designs is a useful tool in order to evaluate the effect of formulation variable on physicochemical properties of solid lipid nanoparticles (SLN). The drug incorporation to SLN were to studied for their physical properties and drug dissolution., To investigate the cytotoxic mechanism and DNA synthesis of various camptothecin formulation in human hepatoma cells（Hep3B and HepG2）. From 25 factorial designs can be found that the main effects of two factors are lipid type and surfactant type on particle size . The CA-SLN had an average diameter of about average 79-119nm.The physical physicochemical state of CA-SLN were examined by DSC, the results indicate camptothecin might exist in an amorphous state in SLN. In vitro results showed that release rate of drug from SLN would be slower than camptothecin solution. SLN might be controlled release delivery system for camptothecin. The efficiency of encapsulation of camptothecin in various nanoparticles was 5-26％. The cytotoxicity of camptothecin solution, camptothecin added with cyclosporin A solution, CA-SLN were determined by MTS assay and flow cytometry. The results indicate that the cytotoxicity of CA-SLN was great on human hepatoma cells（Hep3B and HepG2）.To define the cytotoxic mechanism of camptothecin add with cyclosporin A on human hepatoma cells. The sub-G1 feature analyzed by flow cytometry appeared in 48 hour. Camptothecin solution and CA-SLN treatment might drove the cells at G2/M phase to apoptosis.