Analysis of Ciprofloxacin by a Simple High Performance Liquid Chromatography

• Main Authors: Chih-Yuan Chien, 簡至遠
• Other Authors: Shihn-Sheng Wu
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/73745107892616425130

碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 92 === A simple and sensitive high performance liquid chromatography ( HPLC ) has been described for the quantitative analysis of ciprofloxacin in pharmaceuticals and human plasma. The method employed reversed phase chromatography on a RP-C18 column with an isocratic mobile phase of acetonitrile : 2% acetic acid aqueous solution ( 16 : 84, v/v ) with a flow rate 1.0 mL/min. The UV detector was set at 280 nm. The method was validated in terms of limit of detection ( LOD ), linear range, precision, accuracy, recovery, stability and selectivity. The HPLC method was applied to determine ciprofloxacin in pharmaceuticals ( tablets and injections ). The regression equations were linear between 0.51 ~ 130 μM ( r≧0.9999 ). The relative standard deviation ( R.S.D. ) and relative error ( R.E. ) of intraday and interday were less than 1.52% and 3.00%, respectively ( n = 5 ). The recoveries were greater than 96.6%. The method was also used to determine ciprofloxacin in human plasma. Plasma samples were simply deproteinized with acetonitrile. The regression equations were linear between 0.51 ~ 64.8 μM ( r ≧ 0.9999 ). The R.S.D. and R.E. of intraday and interday were less than 3.39% and 5.71%, respectively ( n = 6 ). The recoveries were all greater than 93.9%. The method had been successfully applied to monitor concentrations of ciprofloxacin on a human volunteer received a single oral dose in a 24 hours period.