Biocompatible Nanoparticles functionalized with Photoluminescent and Superparamagnetic Properties

• Main Authors: CHEN,CHING-JUNG, 陳靖容
• Other Authors: WU, ZHAO-YAN
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/70772225547080751839

碩士 === 義守大學 === 生物技術與化學工程研究所 === 92 === Abstract With an aim to prepare nanoparticles with core-shell morphologies displaying both magnetic and photoluminescent properties, we rendered zinc sulfide thin layers formation on the iron oxide nanoparticle surfaces. Moreover, the core-shell nanoparticles were protected by biocompatible surfactant, 11-mercaptoundecylphosporylcholine (SPC), to extend their applications on biomedical detection uses. On the preparation aspect, the ferrous and ferric salts were coprecipitated under basic condition for the formation of magnetic nanoparticle. After the adsorption of zinc acetate, the zinc sulfide yielded on particle surfaces by ultrasonic irradiation in an aqueous medium. The transmission electron microscope (TEM) image shows the core-shell morphologies of nanoparticle (9 nm of diameter) possibly coated with ZnS thin layers. The optical single absorption peak observed at 265 nm as an indication of the existence of a narrow size distribution of nanoparticles. An emission ZnS band centered at 425 nm (excited at 330 nm) was shown from the photoluminescence spectrum of the nanoparticles. The existence of elements on the surfaces and the inner—outer relationship were evaluated by X-ray Photoelectron spectrum analysis and the occurring of surface reactions was ensured from their chemical shifts. Upon evaluating for biomedical uses, the surfactant-protected nanoparticles mixed with albumin in aqueous buffer solution were analyzed using HPLC separation and TEM observation, concluding a weak interaction between proteins and nanoparticles. Furthermore, the culture of nanioparticles with 3T3 fibroblast cells based on various culturing periods and temperatures showed the particles internalization into cells by fusion and endocytosis pathways from observation by confocal microscope. The results promote the promising potential for the studying tools of cell differentiation and the gene drug carriers.