| Summary: | 碩士 === 大葉大學 === 生物產業科技學系碩士班 === 92 === There was a protease producing microbe isolated from soil at TAIWAN named Bacillus amyloliquefaciens V656. Purification and characterization of protease from B. amyloliquefaciens V656 are targets of the study. The optimized conditions for protease production was found when the culture shaken at 30°C for 2 days in 100 ml of medium (pH value adjusted to 7.0) containing 2% shrimp and crab shell powder (SCSP), 0.1% K2HPO4, 0.05% MgSO4‧7H2O. Under the conditions, the protease of B. amyloliquefaciens V656 attained the activity was 0.15 U/mg.
After purification procedure, the protease from B. amyloliquefaciens V656 shows heat stability and has a molecular weight is 28kDa. The optimum pH value and temperature are pH8 and 37℃.
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