The Study on Cholesterol-Imprinted Thin Film Composite Membranes

• Main Authors: Mei-lien Chung, 鍾美蓮
• Other Authors: Wen-janq Chen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/h79f62

碩士 === 朝陽科技大學 === 應用化學系碩士班 === 92 === The technique to synthesize molecularly imprinted polymer(MIP)involves complexation of template molecule and functional monomer, followed by polymerization of this complex with crosslinker to form a highly crosslinking polymer, MIP exhibits specific recognition ability to template molecule due to the affinity between functional group and template molecule and it’s well-defined shape which allows the template molecule specifically to fit in. The purpose of this study is to synthesize cholesterol-imprinted polymerization, UV(254nm) stimulated polymerization with methacrylic acid (MAA) as functional monomer, divinylbenzene (DVB) and Ethylene glycol dimethacrylate (EGDMA) as crosslinker and 1-hydroxycyclohexylphenyl ketone as initiator. The adsorption determined by analyzing the concentration change of solute in adsorption solution using HPLC. For MIP particles, the results showed that cholesterol-imprinted polymer adsorbed different amount of cholesterol and progesterone when they were placed in the adsorption solution of single solute and in the adsorption solution of binary solute. Cholesterol was more selectivity adsorbed by MIP particle than progesterone. Especially for long-period of adsorption, selectivity characteristics for cholesterol was more significant. This results indicated that the synthesized MIP did possess template site which could recognize cholesterol molecule. In addition, the adsorption amount of cholesterol and selectivity factor for cholesterol were enhanced when the polarty of solvent in adsorption solution was increased. For MIP composite membranes, it was found that both MIP membrane and NIP membrane were still retained on the surface of both Al plate and Al-coated glass after series of treatment including extraction, adsorption and re-extraction. Cholesterol-imprinted polymer membrane adsorbed more cholesterol than stigmasterol. NIP membrane adsorbed about the same amount of both cholesterol and stigmasterol. The amount of cholesterol and stigmasterol adsorbed by NIP membrane was smaller than that adsorbed by MIP membrane. The results suggested that MIP membrane did exhibit the ability to recognize cholesterol. With polymerization solution of 95 mole% DVB, the synthesized MIP membrane showed the better selective characteristics toward cholesterol.