| Summary: | 碩士 === 中山醫學大學 === 營養科學研究所 === 92 === It has been confirmed that TRP1 and TRP2 are the important enzymes involved in eumelanogenesis of melanocytes, and the expressions of TRP1 and TRP2 genes are up-regulated by activation of upstream gene, Mitf. Alpha-Lipoic acid decreased eumelanogenesis by regulating the glutathione content in cells, and reduced the intra/ extracellular melanin content of B16-F1 melanoma cells. Therefore, our study was to evaluate (1) the intra/extracellular melanin biosynthesis of B16-F1 melanoma cells when treated with different concentrations of Alpha-lipoic acid at 72 hours, (2) the expressions of eumelanogenesis related genes, including TRP1, TRP2 and Mitf of B16-F1 melanoma cells when treated with different concentrations of Alpha-lipoic acid at 72 hours. Our results has shown that (1) the intra/extracellular melanin content were decreased by increasing the concentrations of Alpha-lipoic acid. (2) 400μM Alpha-lipoic acid also reduced the expressions of TRP2 and Mitf of B16-F1 melanoma cells, but TRP1 expression remained unchanged. The results suggested that Alpha-lipoic acid inhibited the expression of Mitf, and further inhibited the expression of a downstream gene, TRP2, to decrease intra/ extracellular melanin content of B16-F1 melanoma cells.
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