Analysis of the hypothetical gene XM_027541 in the Human Genome

• Main Authors: Chan-Tein Wu, 吳鎮天
• Other Authors: Ling-Yun Chen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/05734892879120597222

碩士 === 中山醫學大學 === 生物化學研究所 === 93 === Abstract The glandular epithelial cells of the endometrium existed distinct morphology in the menstrual cycle. These differentiates are from the stimulation with many hormone. In our laboratory, we have identified the specific gene expressing in the early proliferative phase or in the secretory phase such as phospholipids scramblase, NUDT9, and XP_027541. We focus on the XP_027541 because it may exixt some important function in regulating the rule change of endometrium. We first purified the 13 kDa protein and sequencing. And then we get the recombinant protein XP_157-114 and XP_2115-217, and immune the rabbit to gain the polyclonal antibody. We found XP_027541 is expression in the transcription level and translation level. From bioinformation we BLAST from NCBI data base, we found the XM_027541 just near 457 base pair to the ATPase classVI type 11A. But in EBI data base, we found XM_027541 were over lapping with the ATPase classVI type 11A exon 30 region. At last we identified that XM_027541 were located in the exon 30 of ATPase classVI type 11A by PCR and sequencing to suguesst that ATPase classVI type 11A may exist the mechanism of RNA alternative splicing.