The Inhibition Effect of Caesalpinia sappan L. on VEGF-induced Proliferation and Migration in human umbilical vein endothelial cells

• Main Authors: Yu-Chi Wei, 魏佑吉
• Other Authors: Su-yin Chiang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/76599960988800072268

碩士 === 中國醫藥大學 === 中西醫結合研究所 === 92 === Cancer is one of angiogenesis-dependent diseases that involve multiple processes of positive and negative regulations in angiogenesis. Recently, anti-angiogenesis has been regarded as a promising strategy for cancer treatment. The wood of Sappan lignum is one of the Chinese Medicines with blood-activating and stasis-eliminating effects. In this study, the effects of Sappan lignum in anti-angiogenesis were examined in human umbilical vein endothelial cells (HUVEC). Our data showed that Vascular endothelial growth factor (VEGF) induced the proliferation of HUVEC in a dose dependent manner. Sappan lignum (25~100μg/ml) significantly inhibited the proliferation of HUVEC induced by VEGF (20 ng/ml). At the concentrations of 25~50 μg/ml, Sappan lignum almost completely blocked VEGF-induced cell proliferation. However, Sappan lignum exhibited overt cytotoxicity at higher doses (> 100 μg/ml). This effect of Sappan lignum is similar to low dose chemotherapeutic agents that pose cytostatic effect and were used as anti-angiogenesis agents. Furthermore, we found that Sappan lignum markedly reduced VEGF (20 ng/ml)-induced migration of HUVEC in a Transwell migration assay. In conclusion, our data suggest that Sappan lignum can markedly inhibit the proliferation and migration-induced by VEGF in HUVEC. Sappan lignum may be an effective herbal drug in anti-angiogenesis. Further investigation is needed to explore its potential use in animal and clinical studies.