Anoectochilus formosanus effective fraction modulated innate immunity in mice

• Main Authors: Hsu, Chih-Fen, 徐致芬
• Other Authors: Lin, Wen-Chuan
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/70100554535812233661

碩士 === 中國醫藥大學 === 醫學研究所 === 92 === Anoectochilus formosanus effective fraction modulated innate immunity in mice Chih-Fen Hsu Institute of Medical Science,China Medical University Anoectochilus formosanus HAYATA (AF), a traditional pharmaceutical herb, had been used in hepatic protection in Taiwan. In this study, we will use the effective fraction of AF (AFEF) to modulate innate immunity including nautral killer (NK) cell cytotoxic assay, macrophage phagocytosis, splenocyte and lymph node subpopulation differentiate, cell proliferation, cytokine secreting assay and serum immunoglobulin assay. Various doses of AFEF administered to BALB/c mice for 2 and 4 weeks. Primary peritoneal macrophage harvest from mice that administered with various concentration of AFEF was direct to activate phagocytosis. On the other hand, AFEF can significant increase cytolysis effect in NK cell (1500 mg/kg) and that might be involved the activation of T helper cell in activating (CD69). AFEF can also significant increased IFN-production from lymph node cells by ConA stimulation for 48 hours in administration AFEF (1500mg/kg). Further study to understand the mechanism of phagocytosis, in vitro AFEF-treated RAW 264.7 cells were analyzed in this study. AFEF can significant increases RAW 264.7 cell phagocytosis (100, 200 g/ml), by increased nitrite, IL-2 and IFN- secretion. Taking all these data together, AFEF might be the active fraction in activation of innate immunity.