Determination of Volatile Fatty Acids by Solid-Phase Microextraction from Trash Wastewater

• Main Authors: Hung Miao Fen, 洪妙芬
• Other Authors: Jinn-Shoung Lai
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/06377973904757801768

碩士 === 中國醫藥大學 === 環境醫學研究所 === 92 === Volatile fatty acids（VFA） are the most common intermediate products generated from anaerobic decomposition of organic substances. This study develops a headspace solid phase microextraction procedure for sampling of VFA. This method is simple, convenient, and fast. It does not require organic solvents, and is not interfered by base materials. It combines the gas chromatograph to determine the analysis of volatile fatty acids contents in the trash wastewater. In this study, 75 μm PDMS/CAR fiber was used. The fiber absorption time, absorption temperature, desorption time and desorption temperature were studied to determine the optimal extraction analysis conditions. The results showed that the best absorb time was 20 minutes at 1200 rpm mixing speed under room temperature. The injection point temperature of the instrument was set at 250℃, with 3 minutes of hot desorption. GC-FID was used for analysis. The linear correlation coefficient of standards acetic acid, propanoic acid, butyric acid, and iso-butyric acid are all greater than 0.995. The detection limits for acetic acid, propanoic acid, butyric acid, and iso-butyric acid were 9.2 μg/ml, 2.65μg/ml, 3.11μg/ml, 2.47μg/ml, respectively. The detection limits were all within ppm range. The precision were lower than 10﹪. The accuracy were in the range of 88.04-112.32﹪. The equipment for solid phase microextraction is simple, easy to operate and is very suitable for field environment sampling. This research selects trash wastewater as real sample to test applicability of this microextraction method. This method may simplify the complex procedure of sampling and sample pre-processing, and lower analysis costs. It is a very practical and effective pretreatment method.