A STUDY OF OXIDIZED LDL-INDUCED VASCULAR ENDOTHELIAL CELL APOPTOSIS PROTECT BY Paeonia suffruticosa Andr. And Carthamus tinctorius L.

• Main Authors: lin mei-yi, 林美儀
• Other Authors: 唐娜櫻
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/89546568609154856363

碩士 === 中國醫藥大學 === 中國醫學研究所 === 92 === The formation of reactive oxygen species (ROS) plays an important role of the development of atherosclerosis because ROS promotes cell proliferation and/or apoptosis and oxidation of LDL. One of the proatherogenic properties of oxidized LDL (OxLDL) is its toxicity toward cells including endothelial cells, smooth muscle cells and macrophages. According to the theoretical system of TCM, atherosclerosis is the disease of the blood stagnation. Paeonia suffruticosa Andr. and Carthamus tinctorius L. usually apply to promoting blood circulation to dissipate blood stasis. Therefore , we hypothesized that Paeonia suffruticosa Andr. and Carthamus tinctorius L. can use to treat atherosclerosis. In this study, we investigated the oxLDL-induced HUVECs apoptosis protect by Paeonia suffruticosa Andr. and Carthamus tinctorius L. First, the cytotoxicity of the two drugs in HUVECs was determinated by MTT assay and the apoptotic of the two drugs was determinated by flow cytometric analysis. Result demonstrated that Paeonia suffruticosa Andr. and Carthamus tinctorius L. induced HUVECs cytotoxity in a concentration-dependent manner. The secondary, oxLDL-induced HUVECs damage was observed by Giemsa stain. When given low concentration of Paeonia suffruticosa Andr. or Carthamus tinctorius L.,the expression of the apoptic body and subG1 induced by oxLDL was reduced. Result demonstrated that Paeonia suffruticosa Andr. and Carthamus tinctorius L.can protect against oxLDL-induced damage. These results suggest that Paeonia suffruticosa Andr. and Carthamus tinctorius L. may be a useful candidate in the treatment of atherosclerosis.