Identification of Radio-resistant Gene by Microarray in Head and Neck Cancer

• Main Authors: Wei Chung Laio, 廖偉成
• Other Authors: Ann Joy Cheng
• Format: Others
• Language: en_US
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/74222162092242710990

碩士 === 長庚大學 === 生化與生醫工程研究所 === 92 === Abstract Head and neck cancer is one of the ten frequent cancer in the world with an estimated over 500,000 new cases being diagnose annually. The overall 5-year survival rate for patients with head and neck cancer is among the lowest of the major cancer, and has not changed during the past two decades. In Taiwan, the incidence of head and neck cancer has become the 7th leading cancer, the 5th leading cancer in male, and is still increasing in the recent years. Since this cancer usually occurs in middle age male, at the high peak of life responsibility, it has tremendous impact of family and society. Radiation therapy is an integral part of the treatment of head and neck cancer. However, local recurrence or tumor persistence after radiation therapy is the major cause of treatment failure. The sensitivity of tumor cells to the cytotoxicity of ionizing radiation is, therefore, the major determinants of local tumor control in patient treated with radiation therapy. However, the molecular factors predicting radiation response have not been extensively studied, far away from clinical application of molecular adjuvant therapy to increase radio-sensitivity. The aim of this study is to identify genetic alterations associated with our established radio-resistant subclones (KB and OEC-M1cells) from Taiwanese local head and neck cancer. The result of cDNA microarray screening demonstrated that RP105 (Ly64) gene was found in both KB and OEC-M1 radio-resistant subclones, following identified with RT-PCR that expressed in KB and OEC-M1 radio-resistant subclones. Transfection of RP105 RNAi in radio-resistant subclones reversed this phenomenon. RP105 expression was correlated to growth and radio-resistance in OEC-M1, but less significant in KBRR cells. However, RP105 gene was more expression in leukocytes than OEC-M1 and KBRR, so this gene maybe plays important roles of radio-resistance in leukocytes.