To evaluate the antiplatelet effect of F-7HQO on human platelet
碩士 === 長庚大學 === 天然藥物研究所 === 92 === A synthetic chemical compound, F-7HQO, concentraction dependently inhibited platelet aggregation stimulated by U46619（2μM）、thrombin（0.1U /ml） 、 arachidonic acid（200 μM）with IC50 values of 0.45±0.08μM , 2.32±0.42μM , 0.03±0.01μM in human washed platelet ,...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2004
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| Online Access: | http://ndltd.ncl.edu.tw/handle/30732490363956383417 |
| Summary: | 碩士 === 長庚大學 === 天然藥物研究所 === 92 === A synthetic chemical compound, F-7HQO, concentraction dependently inhibited platelet aggregation stimulated by U46619（2μM）、thrombin（0.1U /ml） 、 arachidonic acid（200 μM）with IC50 values of 0.45±0.08μM , 2.32±0.42μM , 0.03±0.01μM in human washed platelet , respectively . Also , addition of F-7HQO to platelets that had been aggregated caused a concentraction-dependently disaggregation . F-7HQO also prevented intracellular calcium mobilization and thromboxane formation elicited by the inducers. F-7HQO alone did not induce any increase in cGMP but a substantial increase in cAMP levels in platelets . The combination of F-7HQO and prostaglandin E1 caused a synergistic increase in cAMP levels in platelets . However , the combination of F-7HQO and IBMX had no additive effects on platelet cAMP levels .
These data clearly indicated that the antiplatelet effect of F-7HQO is due to elevate cAMP level.
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