| Summary: | 碩士 === 長庚大學 === 化工與材料工程研究所 === 92 === The medicinal plant is currently one of the hot research topics. Due to the complicated structures of some medicinal materials, most cancer- resisting substances can not be produced by organic synthesis and must be produced by cell culture . Therefore, the objective of this research is to cultivate the plant tissue cells which can secrete an anticancer composition — podophyllotoxin .
The materials used in these experiments include podophyllum pleianthum plants from Yang Ming Shan and the callus and suspension cells of the root , steam and leaf from Sitoul offered by the Development Center of Biotechnology. We disinfect the surface of the explant from Yang Ming Shan, cultivate it on a medium based on B5 basal medium supplemented with 1ppm 2,4-D and 0.1ppm kinetin at dark. Callus can thus be greatly produced. The best induced explant is from leaf and its induced rate can reach 92.5%. HPLC analysis indicates that the podophyllotoxin content of the callus from Yang Ming Shan is higher than that from Sitoul. The callus induced from steam and leaf can produce 0.00367% and 0.00341% (g/g) podophyllotoxin, respectively.
Among the suspension cells of the root, steam and leaf from the Development Center of Biotechnology, this study shows that the suspension cells induced from root contain a higher content of podophyllotoxin than those induced from steam and leaf. The content of podophyllotoxin induced from root can reach 0.002% (g/g ).
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