| Summary: | 碩士 === 國立中正大學 === 化學研究所 === 92 === Abstract
Antrodia camphorata, “niu-chang-chih”, is a unique species of fungus found in Taiwan . Its fruit body has been used as Chinese herb in treatment of various diseases. Recent in vivo studies have revealed that crude extracts of Antrodia camphorata can improve liver function, increase immunity and inhibit the growth of carcinoma. The objective of this research is to identify bioactive compounds from Antrodia camphorata’s mycelia with pharmaceutical effect on human liver cancer cells. We used high throughput method to screen and search for bioactive substances from the crude extract by employing human liver cancer cell based assay, instead of slow and expensive animal testing. At first, we were pleased to find that, when treating Hep 3B (a human liver cancer cell) with crude extracts of mycelia and fruit body of Antrodia camphorata for 48 hr, the IC50 values of 44.25 and 12.22 μg/mL were obtained, respectively. The crude extract of Antrodia camphorata was further fractionated (two times) on a silica gel column. These separated fractions (twelve fractions in total) were individually treated with Hep 3B cells. We found that the fraction 6 (F6) has salient bioactivity with a significant IC50 value of 6.4614 μg/mL. The active ingredient in F6 was purified by both HPLC and TLC and structurally identified by FAB MS, NMR and IR. The compound identified was 1-hydroxy-3-isobutyl-4-[4-(3-methyl-but-2-enyloxy)-phenyl]-pyrrole-2,5-dione (formula: C19H23NO4; molecular weight: 329.16). In the treatment of both Hep G2 and Hep 3B cells for 48 h, this compound gave the IC50 values of 9.903 and 6.388μg/mL, respectively.
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