Study on the chitosan oligosaccharide Modified Planar Glucose Biosensor

• Main Authors: Li-In Lu, 呂俐瑩
• Other Authors: Wen-Chang Chen
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/59737185097809903973

碩士 === 國立雲林科技大學 === 工業化學與災害防治研究所 === 91 === An amperometric glucose biosensor strip was fabricated by coating ferricyanide modified with chitin-oligomer and the enzyme glucose oxidase (GOD) on screen-printed carbon electrodes. Sensor strips without GOD were characterized by cyclic voltammetry (CV) and scanning electron micrograph (SEM). A linear relation between peak current and square root of scan rate where the linear line passes through origin suggests reversible electrochemistry of chitin-oligomer modified ferricyanide. Results from chronopotentiometric (CP) measurements showed that an increase of cathodic limiting current was resulted from an increase of ferricyanide concentration on the electrode surface, which could be attributed to the addition of chitin-oligomer. Moreover, results from chronoamperometric (CA) measurements indicated that pH effect on the background current of the sensor strips could be neglected in the range between 6.0 and 9.0. Furthermore, this glucose-sensing system exhibited high sensitivity, a short response time (< 15 s), and a wide linear response range (0-500 mg glucose/dl).