Analysis of the Population Genetics for Aedes aegypti in Cities of Tainan, Kaohsiung and Taitung in 2002

• Main Authors: Chien-Chung Feng, 馮建中
• Other Authors: Chau-Mei Ho
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/72638611235694891803

碩士 === 國立陽明大學 === 寄生蟲學研究所 === 91 === Aedes aegypti is an important vector for dengue fever (DF) and dengue shock syndrome (DSS). In order to study the density fluctuation of Ae. aegypti, 42, 43, and 60 ovitraps were set in cities of Tainan, Kaohsiung and Taitung, respectively. Surveillance of vector for Tainan and Kaohsiung was carried out from April through December in 2002. Survey in Taitung was made from October to November. Eggs or larvae in each ovitrap were collected weekly and examined in the laboratory until adult emerged. Results of egg examination showed that increase of eggs started from June both in Tainan and Kaohsiung, this indicates that vector density was associated with the precipitation. The peak of eggs of Aedes was found in June in these two cities. The increase of eggs also found to be correlated with the rising of temperature. In Tainan, the number of Ae. aegypti was more than that of Ae albopictus except June to August. However, Ae. aegypti was the dominant species in Kaoshiung in 2002. In Taitung, Ae. aegypti only appear at the early of October in a small number. The genetic properties of Ae. aegypti were also studied for the adult bred from our set ovitraps. The abdominal scale pattern was similar in Ae. aegypti from Tainan and Kaohsiung, and more white scale located at the first segment of the Ae. aegypti collected from (RAPD)-PCR were obtained from 121 mosquitoes using 2 primers. The results of clustering analyses showed that mosquitoes in 7 Taitung. Seventy markers of Random amplified DNA polymorphismsareas of Tainan were mixed populations. A similar phenomenon was also observed in the mosquitoes from 3 areas in Kaohsiung. However, the population in Tainan was able to differentiate from that of Kaohsiung. In addition, Ae. aegypti from Taitung appeared to be a branch coming from population of Kaohsiung but had unique banding pattern. Positive correlation between the density of Ae. aegypti and the DF cases also existed in our study.