The Chondrogenic Potential of Human Bone-Marrow-Derived Mesenchymal Stem Cells

• Main Authors: Yen Shi Kuo, 郭妍希
• Other Authors: Yng Jiin Wang
• Format: Others
• Language: en_US
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/38994781921371374626

碩士 === 國立陽明大學 === 醫學工程研究所 === 91 === Abstract The essence of tissue engineering, which is based on the components of cells, scaffolds and signals, is to mimic tissue. In order to optimize the repair process of bone and cartilage, various biological implants have been developed that involve the use of stem cells. Of these, mesenchymal stem cells are probably most commonly used for tissue regeneration. However, there is only limited knowledge about the differentiation potential of these cells and the undergoing process of chondrogenesis. In the present study, we evaluate the performance of several fibrin glue based materials as cell carriers for the chondrogenic differentiation of adult human bone-marrow-derived mesenchymal stem cells. This study will demonstrate the induction of in vitro chondrogenesis with human bone-marrow-derived mesenchymal stem cells and TGFβ1. Fibrin glue, the main scaffold in this study, is a biocompatible and biodegradable material used for hemostasis cell delivery vehicle. Moreover, we have conducted further studies on various scaffolds made of fibrin glue and hyaluronic acid (HA+FG) or polygalaturonic acid (PGA+FG) for mesenchymal stem cells. By means of RT-PCR, type II collagen and aggrecan genes were detected among the samples of FG, (HA+FG) and (PGA+FG) groups. Our results show that (HA+FG) material, with a large pore size of 150 μm in diameter, is a feasible biocompatible scaffold for chondrogenesis in size-sieved mesenchymal stem cells with 10.0 ng/ml TGFβ1.