Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"

碩士 === 國立陽明大學 === 遺傳學研究所 === 91 === C2H2 zinc —finger proteins (ZFPs) constitute the largest family of nucleic acid binding factors in higher eukaryotes. I identified 91putative ZFPs that contain an N-terminal conserved region (LING domain) in the Drosophila melanogaster genome. Some other subfamili...

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Main Author: 許紹君
Other Authors: 簡正鼎
Format: Others
Language:en_US
Published: 2003
Online Access:http://ndltd.ncl.edu.tw/handle/17421700548987364884
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spelling ndltd-TW-091YM0004980192015-10-13T13:39:19Z http://ndltd.ncl.edu.tw/handle/17421700548987364884 Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger" 全新之含鋅指蛋白家族之定義與家族成員之一Littlefinger在果蠅發育中的功能分析 許紹君 碩士 國立陽明大學 遺傳學研究所 91 C2H2 zinc —finger proteins (ZFPs) constitute the largest family of nucleic acid binding factors in higher eukaryotes. I identified 91putative ZFPs that contain an N-terminal conserved region (LING domain) in the Drosophila melanogaster genome. Some other subfamilies of structurally related ZFPs have been defined on the basis of highly conserved N-terminal domains including FAX (finger-associated box) domains found in a large number of Xenopus and KRAB (Kruppel-associated box ) and FPB (finger-preceding box) domains identified in human, mouse, and Xenopus. The LING domain does not correspond to any one of these defined domains. The LING domain-containing proteins distribute in clusters in the Drosophila genome and appear to be restricted to closely related insect genomes. According to the yeast two-hybrid assay, one of these ZFPs Little finger (Lif) could interact with Numb, a key protein involved in neural development. Lif could interact with Numb and Tramtrack (Ttk) through the cooperation of the N-terminal LING domain and the C-terminal zinc finger domain. The lif gene is expressed in the nervous systems of embryonic and third instar larval stages. Misexpression of lif during the formation of sensory organ precursor (SOP) cells increases the number of sensory bristles. Lif interacts with Ttk, a downstream effecter in the Numb pathway. Lif is a nuclear-localized protein implying that Lif acts as a transcription factor in the nucleus. It had been shown that Ttk could repress G2/M phase transition by binding to the promoter region of string, a fly homolog of cdc25. These results illustrate that lif might act as a transcription factor in the proliferation of the nervous system. 簡正鼎 2003 學位論文 ; thesis 0 en_US
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language en_US
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description 碩士 === 國立陽明大學 === 遺傳學研究所 === 91 === C2H2 zinc —finger proteins (ZFPs) constitute the largest family of nucleic acid binding factors in higher eukaryotes. I identified 91putative ZFPs that contain an N-terminal conserved region (LING domain) in the Drosophila melanogaster genome. Some other subfamilies of structurally related ZFPs have been defined on the basis of highly conserved N-terminal domains including FAX (finger-associated box) domains found in a large number of Xenopus and KRAB (Kruppel-associated box ) and FPB (finger-preceding box) domains identified in human, mouse, and Xenopus. The LING domain does not correspond to any one of these defined domains. The LING domain-containing proteins distribute in clusters in the Drosophila genome and appear to be restricted to closely related insect genomes. According to the yeast two-hybrid assay, one of these ZFPs Little finger (Lif) could interact with Numb, a key protein involved in neural development. Lif could interact with Numb and Tramtrack (Ttk) through the cooperation of the N-terminal LING domain and the C-terminal zinc finger domain. The lif gene is expressed in the nervous systems of embryonic and third instar larval stages. Misexpression of lif during the formation of sensory organ precursor (SOP) cells increases the number of sensory bristles. Lif interacts with Ttk, a downstream effecter in the Numb pathway. Lif is a nuclear-localized protein implying that Lif acts as a transcription factor in the nucleus. It had been shown that Ttk could repress G2/M phase transition by binding to the promoter region of string, a fly homolog of cdc25. These results illustrate that lif might act as a transcription factor in the proliferation of the nervous system.
author2 簡正鼎
author_facet 簡正鼎
許紹君
author 許紹君
spellingShingle 許紹君
Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"
author_sort 許紹君
title Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"
title_short Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"
title_full Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"
title_fullStr Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"
title_full_unstemmed Identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "Little finger"
title_sort identification of a novel family of zinc-finger proteins and functional characterization of one of the family member "little finger"
publishDate 2003
url http://ndltd.ncl.edu.tw/handle/17421700548987364884
work_keys_str_mv AT xǔshàojūn identificationofanovelfamilyofzincfingerproteinsandfunctionalcharacterizationofoneofthefamilymemberlittlefinger
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