Immunocytochemical Reactivity and Morphological Changes of the Astrocyte in the Cerebrum of Gerbils after Heat Shock and Hyperthermic Treatment

• Main Authors: Wei - Hui Chiang, 江偉慧
• Other Authors: Shang-Ming Yu
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/25992314728218725810

碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 91 === Pretreatment of the heat shock under the environmental stress induced neuronal protection from the stress insult and maintained the internal homeostasis. Gerbils were used in this study for heat shock and hyperthermic treatment. The heat shock experiment was processed under the 420C for 30 min (H0) and recovered for 24 hours (H24h). Thereafter, the hyperthermic treatment was processed under 450C for 60 min (H24hS0) and recovered for 4 (H24hS4h), 18 (H24hS18h) and 24 hours (H24hS24h). The striking change of brain area as following：methylene blue stained cells changed from cuboidal shape to elongated at H0 in the ependymal cell layer of the lateral ventricle. In the corpus callosum, methylene blue stained cells became intensely and evidently at H24hS4h. In the internal capsule, methylene blue stained cells became hypertrophy, spindle-shaped at H24hS0 and more intensely stained at H24hS4h. In the corpus striatum, methylene blue stained cells were intensely stained at H0. In the AVDM of thalamus, methylene blue stained cells became hypertrophy at H24h. In the LSD of thalamus, methylene blue stained cells became lightly stained and transparent at H24hS0. In the ependymal cell layers of the lateral ventricle, GFAP-like-IR was found moderately in the controls, decreased at H0, returned to that of the controls at H24h, remained unchanged at H24hS0, decreased at H24hS4h, increased at H24hS18h similar to that of the H24 from the insult, and increased markedly at H24hS24h. In the corpus callosum, GFAP-like-IR was not discernible in the controls, remained unchanged from H0 through H24h, increased moderately at H24hS0, became not discernible at H24hS4h, increased markedly at H24hS18h, and decreased slightly at H24hS24h. In the internal capsule, GFAP-like-IR was not discernible in the controls, remained unchanged at H0, increased moderately and diffusely at H24h, decreased slightly at H24hS0, became not discernible at H24hS4h, increased slightly at H24hS18h similar to that of the H24hS0, and main found in the nerve process at H24hS24h. In the corpus striatum, GFAP-like-IR was found moderately in the soma and nerve process of the controls, retained in the nerve process at H0, remained unchanged at H24h, increased markedly at H24hS0, became not discernible at H24hS4h, increased moderately similar to that of the controls at H24hS18h, and became only retained in the nerve process at H24hS24h. In the AVDM of thalamus, GFAP-like-IR was not discernible in the controls, remained unchanged at H0, became clumped and increased intensely at H24h, increased slightly at H24hS0, became only retained in the nerve process at H24hS4h, became diffusely to surround the soma at H24hS18h, and became not discernible similar to that of the controls at H24hS24h. In the LSD, GFAP-like-IR was located around the capillaries in the controls, became not discernible at H0, increased markedly at H24h, became moderately at H24hS0, decreased markedly at H24hS4h, became not discernible at H24hS18h similar to that of the H0, and remained unchanged at H24hS24h. The present findings indicated that GFAP may be involved in manipulating the micro-environmental homeostasis and protection for cell survival from the heat insult.