Interaction and Mechanism of Lipopolysaccharide with Chitosan and SACCHACHITOSAN

• Main Authors: Li-Haw Lo, 羅力豪
• Other Authors: Ching-Hua Su
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/86640958780495927666

碩士 === 臺北醫學大學 === 醫學研究所 === 91 === The purpose of the present study was to elucidate the interaction between lipopolysaccharide (LPS) derived from Salmonella minnesota (Re 595) and chitosan as well as SACCHACHITOSAN, a chitosanous fraction from a medicinal mushroom, Ganoderma. Mixtures in different ratio of LPS and several commercially available chitosans in molecular weights or SACCHACHITOSAN were used for investigation. The spectra of SDS-PAGE showed that LPS was arrested at original position through increase of chitosan or SACCHACHITOSAN ratio that resulted in disappearing of LPS bands visualized by silver staining. The measurement of surface potential for the mixtures by Zeta-90 demonstrated that a highest level occurred at the ratio of 8:1, 6:1, 2:1 and 2:1 for low molecular weight, medium molecular weight, high molecular weight chitosans and SACCHACHITOSAN to LPS, respectively. Similar results were also obtained from the analysis with size-exclusion high performance liquid chromatography (SE-HPLC) that the peak shift of the mixtures of chitosans and LPS was in good agreement to the difference of calculated molecular weight. In vitro test in the culture of RAW264 macrophage cell line revealed that nitrogen oxide (NO) production of LPS was inhibited by the adding chitosans or SACCHACHITOSAN. The binding effect of the positive charged chitosan to LPS could provide a good explanation to the inhibition effect of gram-negative bacteria growth in the tissue of the wound when chitin material was applied as wound dressing. It might be also useful for the patients of sepsis by a possible treatment of dialyzing peripheral blood through a chitosan or chitin rich biomaterials.