The Detection of One Specific DNA Molecule

碩士 === 國立臺北科技大學 === 化學工程系碩士班 === 91 === This thesis focuses on two parts:the major one is to detect one specific DNA molecule and the other is to confer the conformation of a variety of plasmid DNA. To improve the sensitivity of DNA detection, we have developed an electrical detection of...

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Main Authors: Ker-Ciang Chen, 陳克強
Other Authors: Shao-Yi Hou
Format: Others
Language:zh-TW
Published: 2003
Online Access:http://ndltd.ncl.edu.tw/handle/05097905125448628006
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spelling ndltd-TW-091TIT000630152015-10-13T13:35:31Z http://ndltd.ncl.edu.tw/handle/05097905125448628006 The Detection of One Specific DNA Molecule 單一特定DNA分子之偵測 Ker-Ciang Chen 陳克強 碩士 國立臺北科技大學 化學工程系碩士班 91 This thesis focuses on two parts:the major one is to detect one specific DNA molecule and the other is to confer the conformation of a variety of plasmid DNA. To improve the sensitivity of DNA detection, we have developed an electrical detection of one specific DNA molecule in this study. The target single strand DNA containing thiol was fixed onto one of gold-microelectrodes. By applying electric field, this ssDNA was straightened and linked the two gold-microelectrodes. A DNA-Au bridge was then formed by using several appropriate ssDNA-Au nanoparticle probes which are complementary to different regions of the target ssDNA. The conductivity measurement was made after Ag filled the gap among the gold nanoparticles. The results reveal that this strategy is successful to target ssDNA containing thiol fixed onto each of gold-microelectrodes. The value of electric current increases when the reduction time of Ag particles increases. Judged by SEM image, three specific DNA molecules were detected through electric current measurement. Based on our calculation, one specific DNA molecules was detectable through this system. This study used TM-AFM to observe the variations in DNA conformation. We attempted to set up different conditions to find out the difference of conformation. It is well-known that plasmid DNA is coil structure in natural condition. However, this study discovered that the plasmid DNA is capable of forming a network with Mg2+and Ni2+ at appropriate concentration. The linear DNA obtained by restriction enzyme cut was arranged along the direction of water flow. Shao-Yi Hou 侯劭毅 2003 學位論文 ; thesis 98 zh-TW
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language zh-TW
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description 碩士 === 國立臺北科技大學 === 化學工程系碩士班 === 91 === This thesis focuses on two parts:the major one is to detect one specific DNA molecule and the other is to confer the conformation of a variety of plasmid DNA. To improve the sensitivity of DNA detection, we have developed an electrical detection of one specific DNA molecule in this study. The target single strand DNA containing thiol was fixed onto one of gold-microelectrodes. By applying electric field, this ssDNA was straightened and linked the two gold-microelectrodes. A DNA-Au bridge was then formed by using several appropriate ssDNA-Au nanoparticle probes which are complementary to different regions of the target ssDNA. The conductivity measurement was made after Ag filled the gap among the gold nanoparticles. The results reveal that this strategy is successful to target ssDNA containing thiol fixed onto each of gold-microelectrodes. The value of electric current increases when the reduction time of Ag particles increases. Judged by SEM image, three specific DNA molecules were detected through electric current measurement. Based on our calculation, one specific DNA molecules was detectable through this system. This study used TM-AFM to observe the variations in DNA conformation. We attempted to set up different conditions to find out the difference of conformation. It is well-known that plasmid DNA is coil structure in natural condition. However, this study discovered that the plasmid DNA is capable of forming a network with Mg2+and Ni2+ at appropriate concentration. The linear DNA obtained by restriction enzyme cut was arranged along the direction of water flow.
author2 Shao-Yi Hou
author_facet Shao-Yi Hou
Ker-Ciang Chen
陳克強
author Ker-Ciang Chen
陳克強
spellingShingle Ker-Ciang Chen
陳克強
The Detection of One Specific DNA Molecule
author_sort Ker-Ciang Chen
title The Detection of One Specific DNA Molecule
title_short The Detection of One Specific DNA Molecule
title_full The Detection of One Specific DNA Molecule
title_fullStr The Detection of One Specific DNA Molecule
title_full_unstemmed The Detection of One Specific DNA Molecule
title_sort detection of one specific dna molecule
publishDate 2003
url http://ndltd.ncl.edu.tw/handle/05097905125448628006
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