Improvement of Gluconobacter oxydans by using technique of conjugal mutation and gene recombination

• Main Authors: Hui-chen Hsiao, 蕭惠真
• Other Authors: Ken-Yuon Li
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/02201132707801301239

碩士 === 東海大學 === 食品科學系 === 91 === The objective of this study is to genetically modify a gluconate producing strain of Gluconobacter oxydans (Henneberg) De Ley (ATCC23771). First, the strain was mutated by conjugative transposon Tn5, and then screening higher yield mutants was performed. Some mutants with higher gluconate production were selected. Mutants, m3, m6 and m9 are approximately 30% higher than the parent strain. The wild type strain of G. oxydans is not saccharolytic. Among the industrial starch hydrolytic enzymes, amylopullulanase (apu) is a powerful one. The apu gene of plasmid TOEAN 1C was contructed in the plasmid pLKY203. The apu gene of this plasmid is successfully expressed by E. coli JM105. Howere, the apu gene is limitedly expressed by Gluconobacter oxydans ATCC23771.