Determination of Selenium in Pig Liver and Pig Kidney by Electrothermal and Flow Injection Hydride Generation Atomic Absorption Spectrometry

• Main Authors: Yat-Sen Sun, 蘇庭瑤
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/34717747767544406678

碩士 === 靜宜大學 === 應用化學研究所 === 91 === In this research，graphite furnace and flow-injection atomic absorption spectrometry for the determination of selenium in pig liver and pig kidney from market were established. The bovine liver SRM 1577b were determinated to identify the accuracy. T-test were used to confirm the system errors between GFAAS and FIAS-HGAAS methods. When GFAAS was used to determine selenium in samples. Selenium has low pyrolysis temperature (<500℃) and biological samples content a lot of proteins matrix. When proteins heating，the carbide was residued in graphite tube and cause high background absorption. After used ascorbic acid and Pd(NO3)2 mixture modifiers not only increased pyrolysis temperature to 1000℃ but also improved the selenium absorption and reduced background absorption. The established method to determine bovine liver SRM 1577b can also have good accuracy. When selenium in samples was determinated by FIAS-HGAAS. HNO3 was used as digestion solution. Simultaneous content Se (VI) and Se (IV) and had different hydride effect. Thus，the pre-reduced Se(VI) to Se(IV) process was applied to the determination with good accuracy results. In this research，the analytical results were closed and good enough to certified value. The recovery were in 92~105%. The D.L. was 27.6pg in GFAAS and 0.144ppb in FIAS-HGAAS. The linear range was 0.1~2ng with GFAAS and 3~20ppb with FIAS-HGAAS. The content of Se in pig liver was 2.42 ±0.15 (g / g) and 2.21 ±0.16（μg / g），respectively. The content of Se in pig kidney was 8.03 ± 0.55 (μg / g) and 8.41 ± 0.44（μg / g），respectively. There were without certified values. Thus t-test was used to compared with two methods，and the results of t values < 4.6（N=5）were obtained. In this research，there were no system errors between two methods，and good accuracy and precision were obtained.