Effect of deoxyribonucleic acid and nucleoprotein on brain and immunity function in senescence accelerated mice.

• Main Authors: Fang-Yi Lin, 林芳儀
• Other Authors: Ming - Fu Wang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/40825498990523372668

碩士 === 靜宜大學 === 食品營養研究所 === 91 === Abstract Many health problems usually accompany aging. Due to the increase of population and a prolonged life span, the aging related issues have brought great attention to the world and have been discussed extensively. During the aging process, it is believed that aging causes the deterioration of the central nervous system and lowers immunity function. The free radicals theory of aging postulates that free radicals reactions cause the DNA damage, cause aging and brain retrograde, and effect of learning and memory ability. The purposes of this study were to examine the effect of deoxyribonucleic acid (DNA) and nucleoprotein supplements on brain and immunity function in senescence accelerated mice (SAMP8). Six-month-old mice were divided into four groups: casein diet group (control group) and casein diet supplemented with 0.2%, 0.5%DNA and 0.8%nucleoprotein. After 12 weeks feeding, body weight, food intake, aging score, open field activity test, single-trial passive avoidance and active shuttle avoidance test were performed during the experiment. After the experiment, the mice were sacrificed to analyze the biochemical parameters, total antioxidant equivalent of serum, activities of superoxide dismutase (SOD) in the liver and in the brain and to evaluate the brain histopathology of spongy degeneration and lipofuscin. The proliferation of spleen cell and Th1 cytokine released IFN-γ. The results showed that food intake, body weight and locomotion were no significantly among four groups. The aging score of the control group was higher than the experimental group (P<0.05). In learning and memory, the male mice fed with 0.2%, 0.5%DNA and 0.8%nucleoprotein had significantly better single-trial passive avoidance test (P<0.05). However, in female 0.2% and 0.5%DNA groups had better learning and memory ability after the first 24 hours. In active shuttle avoidance test, it showed male and female mice had significantly better learning and memory ability. Male mice fed 0.5%DNA had lower total cholesterol and low density lipoprotein cholesterol than the other groups. However, BUN, creatinine and uric acid were no significant differences. Total oxidant equivalences of serum were higher in male and female experimental groups. The 0.2% DNA group had higher activity of SOD in the liver than control group; 0.2%, 0.5%DNA and 0.8%nucleoprotein had better activity of SOD in the brain. The spongy degeneration and lipofuscin of brain in the DNA and nucleoprotein groups were lower than the control group. The mice fed with 0.2%, 0.5%DNA and 0.8%nucleoprotein group had no significant differences in proliferation of spleen cell and Th1 cytokine released IFN-γ. In summary, we conclude the supply of DNA and nucleoprotein may improve aging score, learning and memory ability, reducing the brain pathological changed in mice, and also could promote the antioxidative system. However, the effect of DNA and nucleoprotein in the immunity function need more studies to illustrate in the future.