Effective High-throughput Identification on The Global Cytoplasmic Membrane Protein-bound Oilgopeptides

• Main Authors: Sung Fan Chung, 宋範強
• Other Authors: Chun-Fan Chang
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/68585291042463457148

碩士 === 中國文化大學 === 生物科技研究所 === 91 === Abstract This research establishes the high-throughput screening ( HTS ) technology platform to operate selection of the candidate oligopeptide at automatic equipments and high speed processes. The candidate oligopepetide can become the lead drug by employed binding to cytoplasmic membrane protein, because many studies show the important art of cytoplasmic membrane proteins in cancer cells consider with apoptopsis or signal transduction. But then we integrated the combinatorial library which synthetic randomly tri-, tetra-, and penta-oligopeptides via the strategy of mix-and-select method in times before. It is better using combinatorial chemistry is advances in drug discovery than find complex mixture of natural product such as plant and animal extracts in traditional drug discovery. So we really find some of acute myeloid leukemia ( AML ) of cytoplsmic membrane protein-bound oligopeptides such as HER, SGMR, and NLGR etc. binding to AML's bone marrow specimen and TMR, VAVK, DGNK etc. binding to AML's peripheral blood lymphocyte specimen could become important anti-cancer agents. In the last few years, the human genomic project increase annotation of the genome functions. But all of know protein that is the genetic product process the cell growth and metabolism. This research focus to integrate proteomic technology, because of it is big engineering to analye the 10,000 — 20,000 proteins expressed in a mammalian cells. However, proteomic and mass manipulation provide simple and convenient methods which can review the profile of the mammalian cell proteome. In this study, we firstly assess two-dimensional gel electrophorsesis ( 2-DE ) of cytoplasmic membrane protein variability of the AML in pretreatment and posttreatment by chemical therapy. We hope find specific spots on the 2-DE for future progess in theranostic agents. Now we can to do appling the candidate oligopeptides screen these specific spots which seek to binding correlation between response to specific candidate, and maybe will lucrative new drugs or diagnostics probes.