| Summary: | 碩士 === 中國文化大學 === 生物科技研究所 === 91 === The digesting, transformation, absorption, transport and utilization, of chlorophylls and their physiological function in animals are still a myth. It is worthy to study if chlorophyll- or porphyrin-related compounds (CRCs or PRCs) play any role in animal. In this study, the red fluorescent protein (RFP) of silkworm (Bombyx mori) was employed to explore the above question. RFP has been known to be antivirus in the digestive juice of silkworm. Absorption spectrophotometer, high performance liquid chromatography, Thornber and MARS electrophoresis system and western blotting were hired to analyze the biochemical characteristics of mulberry leaf、RFPI in the digestive juice, and RFPII on the digestive tract.
Data show that mulberry leaf、RFPI and RFPII are very different. By using a reversed-phase HPLC system chlorophyll a and b (Chl a and b) were found in RFPI purified according to Hou and Chiu (1986). The result is different from that of Hayashiya (1978) and Uchida and Hayashiya (1981), who reported that RFPI contained only chlorophyllide a (Chlide a).The Thornber and MARS electrophoresis systems and western blotting show both RFPI and RFPII contain four apoproteins of pigment-protein complexes of mulberry leaf, including RCIa、LHCIIa、LHCIIb and LHCIIc.
Combination of the above data suggest that pigments and proteins of RFP I and RFPII might be totally or partially and directly or indirectly tranformed from pigment-protein complexes responsible for photosynthesis in mulberry leaf. It is therefore suggested that there may be a new model of assimilate nutrients in silkworm larvae.
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