| Summary: | 碩士 === 國立臺灣大學 === 口腔生物科學研究所 === 91 === Inflammatory reactions against invaders in the body call upon cytokine molecules that elicit systemic responses, such as fever, fatigue, increased pain sensitivity and appetite loss, mediated by the central nervous system. For example, systematic invasion of lipopolysaccharide (LPS) , a bacterial endotaxin, produced severe pathological responses in host through the humoral actions of the immune factors (e.g. TNF-α, IL-1). The present study investigated whether the distribution and/or intensity of immunolabeling for Fos in the barin stem differ after imposition of endotoxin of variable doses. Vehicle (0.9% NaCl) or different doses of LPS (42 ng, 56 ng, 70 ng, 140 ng in 0.9% NaCl) were delivered directly into the DVC of adult male Wistar rat. In order to identify some of the molecular mechanisms that occur after a central nervous system injection, the immediate early gene encoding protein Fos was analysed by immunocytochemistry following unilateral (left) vehicle or LPS injection (controls or 30 min, 1h, 2h LPS post-injection). In the section of Interaural -4.80 mm，Bregma -13.80 mm, c-Fos proteins were induced in AP and bilateral NTS at 30 min post-LPS-injection (70 ng, 140 ng in 0.9% NaCl), massively expressed at 2 h post- LPS-injection. In the section of Interaural -0.80 mm, Bregma -9.80 mm, Fos proteins were induced in locus coeruleus, Nucleus Raphe Magnus and lateral superior olivary complex at post-LPS-injection more than control. These data indicated that LPS injection into the dosal vagal complex leads to a rapid and widespread induction of c-Fos. The alterations in immediate early gene (c-Fos proteins) expression reported here may be due to changes in neuronal activity by endotoxin.
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