Isolation and application of thermophiles from bagasse compost

碩士 === 國立臺灣大學 === 環境工程學研究所 === 91 === Abstract It is difficult to decompose cellulose in composting for agricultural waste. Proper microorganisms are commonly added to accelerate compost maturity. In this study, 16 thermophiles with ability to hydrolyze cellulose are isolated from bag...

Full description

Bibliographic Details
Main Authors: Yu, Yi-Hao, 郁儀豪
Other Authors: 張慶源
Format: Others
Language:zh-TW
Published: 2003
Online Access:http://ndltd.ncl.edu.tw/handle/76563501985665325508
Description
Summary:碩士 === 國立臺灣大學 === 環境工程學研究所 === 91 === Abstract It is difficult to decompose cellulose in composting for agricultural waste. Proper microorganisms are commonly added to accelerate compost maturity. In this study, 16 thermophiles with ability to hydrolyze cellulose are isolated from bagasse compost. There are 11, 3 and 2 isolates of actinomycetes, bacteria and fungi, respectively. The selecting conditions consider the temperature tolerance of enzyme and completion of enzyme system. The final identification is then made. The results indicate that isolates of bacteria, actinomycetes and fungi are Bacillus subtilis and Bacillus licheniformis, Streptomyces thermocarboxydus and Streptomyces thermocoprophilus, and Aspergillus fumigatus Fresen. The effect of adding different microorganisms on the acceleration on the compost decomposition are examined. Compost materials used are bagasse and soybean dregs. Cases without inoculation and with inoculating bioreagent are contrast. The results indicate that adding thermophilies can accelerate the compost maturity. Inoculation of mixed S. thermocarboxydus and S. thermocoprophilus shows the best performance, significantly decreasing the time for stabilization of compost. It needs only 12 days to meet the standard of maturity. Inoculating A. fumigatus Fresen needs 14 days to achieve maturity. These two inoculation methods are better than that with bioreagent. Thus, they have possible potentials for producing bioreagent for future use. Keywords: compost, cellulose, cellulase, thermophiles