| Summary: | 碩士 === 國立臺灣大學 === 農業化學研究所 === 91 === The aim of this study was to produce cellulases and to enrich protein of Pangolagrass by solid-state fermentation (SSF). Screen by Congo Red test, strains with high cellulase activity and growth rate on CMC medium were selected for further SSF. Sterilization time, moisture content, nitrogen source, phosphate source, incubation temperature, organic materials, and trace element content were evaluated by SSF to enrich protein and produce cellulase.
In Congo Red test, isolates with large value of colony size, and high ratio of clear zone to colony size were picked and Trichoderma reesei QM9414 was used as reference strain (colony size 21.0±1.22mm and clear zone/colony size 1.55±0.10) at 30℃for 6 days. Among 100 isolated strains, 36 expressed high cellulase activity and growth rate on CMC medium were further screened by medium with Pangolagrass as only carbon source. Twenty four isolated strains, including 3 bacteria, 5 actinomyces, and 16 fungi, were selected for further SSF study. The results showed that isolate CL3 produced high cellulase activity and protein content during fermentation.
The optimal conditions for cellulase production and protein enrichment of Pangolagrass with SSF by isolated strain CL3, were Pangolagrass medium at an initial moisture content of 68~70%, pH 6.5~7.0, supplemented with 2.5% nitrogen source at 35℃for 6 days to give a product of crude protein 16.96%. After solid state fermentation by isolate strain CL3, each gram of substrate produced 3.762 U avicelase, 4.400 U CMCase, and 4.134 U β-glucosidase (dry weight basis). The in vitro digestion of fermentation product were improved from 4.29% to 17.8
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