Study on the anti-tumor effect of fungal immunomodulatory protein FIP-fve from Flammulina velutipes, and its mechanisms

• Main Authors: Yuan-Ping Tu, 杜元平
• Other Authors: Fuu Sheu
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/62066788825526437769

碩士 === 國立臺灣大學 === 園藝學研究所 === 91 === Abstract The objective of the present study was to investigate the anti-tumor effect and its related mechanisms of FIP-fve. The purified FIP-fve, which was obtained from the fruiting bodies of Flammulina velutipes, agrees well with the finder of this protein in its molecular weight determined by SDS-PAGE. Its immunomodulatory activity toward murine splenocytes was also confirmed according to the assays for cell proliferation and interferon -production. Oral administration of FIP-fve (10 g protein/g mouse) significantly increases the life span of ATCC BNL 1MEA.7R.1 hepatoma-bearing mice (1x106、1x105、3x104 cells/mouse) by 11 %、45.7 and 42.2 %, respectively (p < 0.05). The result suggests that FIP-fve displays activities to suppress hepatoma growth in vivo. To understand the mechanism corresponding to this anti-tumor activity, effects of FIP-fve on activating the non-specific and specific immunity of the host were further investigated. Results show that oral administration of FIP-fve can activate peritoneal macrophages. The TNF-and nitric oxide productions in the cultured soup of peritoneal cells, which were obtained from FIP-fve fed mice, were significantly increased to those of the PBS fed control (p < 0.05), whether these cells were activated by LPS or not. Additionally, the tumoricidal activity of peritoneal cells obtained from FIP-fve fed mice was also enhanced (p < 0.05). Furthermore, oral administration of FIP-fve also activates the specific immunity of the host. In hepatoma-bearing mice, the tumor-specific IgG levels in serum were markedly increased to the control (p < 0.05). When the splenocytes, which were obtained from FIP-fve fed hepatoma-bearing mice, were treated with the tumor cells in vitro, their tumor-specific proliferation and IFN- / TNF- production were greatly promoted (p < 0.05). Moreover, these splenocytes also showed tumor-specific tumoricidal-activity (p < 0.05). These results suggest that FIP-fve activates immune cells in a wide spectrum. In hepatoma-bearing mice, B cells were activated to produce more antibodies against tumor. Spleen cells were activated in proliferating and secreting cytokines such as IFN- / TNF-. Tumor-specific Tc cells were also activated and differentiated into CTLs, which show potent tumoricidal-activity. Putting into together, FIP-fve shows anti-tumor activity by activating both non-specific and specific immunity of its host. It is helpful for further understanding the nature effects of FIP-fve on the human body and other medicinal applications.