Garlic oil compositions of 24 garlic lines cultivated in Taiwan and their effects on the cell viabilities of human liver tumor cell HepG2 and normal primary rat hepatocytes

• Main Authors: Li,Yun Liao, 廖麗雲
• Other Authors: Lee-Yan Sheen
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/26567716684609248421

碩士 === 國立臺灣大學 === 食品科技研究所 === 91 === Abstract The objectives of this study was to investigate the effects of twenty-four lines garlics included two kinds of type which were hard-neck and soft-neck from Taiwan. We extracted garlic oil (GO) by steam distillation method, and the average extraction were around 0.2%. The highest extraction (0.26%) was Chia-yi lines of hard-neck type. The components of twenty-four lines garlic oils were analyzed by GC-MS and used Cluster analysis method to analysis the five major organosulfur components (OSCs) which were diallyl sulfide (DAS), diallyl disulfide (DADS), methyl allyl disulfide (MADS), diallyl trisulfide (DATS), and methyl allyl trisulfide (MATS) to get four groups. The types of four groups were chosen hard-neck type of black leave, Chia-yi, white leave of Si-luo, and soft-neck type of An-nan flower to be sampling, and used the four samples to observe the effect of cell viability of the cell line HepG2 from human liver tumor cell and normal primary SD rat hepatocytes. The lowest IC50 effects of garlic oil concentraction for HepG2 from human liver tumor cell viability was black leave, and then were white leave of Si-luo and Chia-yi lines. The garlic oil from An-nan flower had the highest IC50 effects. The IC50 effects of garlic oil concentraction for normal primary SD rat hepatocytes, the garlic oil from white leave of Si-luo had the lowest effect, and then were black leave and Chia-yi lines. The garlic oil from An-nan flower had the highest IC50 effects. In a word garlic oil had lowet IC50 effects for HepG2 cell viability than normal primary SD rat hepatocytes.