| Summary: | 碩士 === 國立臺灣大學 === 化學工程學研究所 === 91 === Abstract
Chitosan is a biocompatible, antiseptic, widely available and inexpensive natural biomaterial. It was extensively used in biomedical engineering field. However, chitosan had low biodegradability and its preparation method (lyopholization) was time consuming thus limiting its further application. To enhance the degradation rate of chitosan, lysozyme was used to disintegrate chitosan matrices. Further, the freeze-gelation method instead of lyopholization was utilized to prepare porous chitosan matrices with short prepared time. Our results indicated that the porosity (above 80%), swelling ratio (12~15), hydrophilic and degradation rate of chitosan matrices by freeze-gelation method were better than those prepare by traditional lyopholization method. The SEM micrographs also revealed that the chitosan matrices prepared by freeze-gelation method were symmetric and porous. The results of matrices degradation by lysozyme suggested that different ways of adding lysozyme to the matrices led to altered initial degradation rates (the first 4 days). The degree of degradation reached 20% for long incubation time (more than 4 days). Therefore, the degradation rate of chitosan matrices can be adjusted by using different lysozyme concentrations or different ways of adding lysozyme to the matrices.
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